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The C464A mutation disrupts a metal-bindingsite in the RING domain, thereby eliminating the MDM2 E3 ligase activity.Compounds that specically increase the luminescence of MDM2 (wt)-luciferasefusion protein rather than MDM2 (C464A) would likely inhibit MDM2 E3 ligaseactivity or proteasomal degradation of MDM2. An alter-native method is to use a urine bag and aspirateurine with a ?ne catheter from the bag as soon asthe urine is voided (Hartnoll et al. 2012)? Is an interbodyfusion needed? Should the procedure be done as an outpatient procedure or shouldthe patient be admitted (Purzner et al.

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